recombinant human bag3 proteins  (ProSpec)

Journal: Antioxidants

Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

doi: 10.3390/antiox9080679

Figure Lengend Snippet: Mesenteric vascular Western blot graph ( A ) and analysis of 4-HNE ( B ), CaSR ( C ), p-eNOS ( D ) and BAG3 ( E ) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F ) and vasodilator (acetylcholine, Ach, G ) is displayed in CON and STZ groups. All data are presented as the mean ± SEM ( n = 3) using the single values in each test. * p < 0.05 vs. CON group.

Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

Techniques: Western Blot

Journal: Antioxidants

Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

doi: 10.3390/antiox9080679

Figure Lengend Snippet: Western blot and immunohistochemistry of BAG3 ( A , B ) and Bcl-2 ( C , D ) in IR myocardium. BAG3 and Bcl-2 expression is significantly decreased in the STZ group than those in the CON group. The BAG3 and Bcl-2 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 and Bcl-2 expression than IR CON hearts. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON.

Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

Techniques: Western Blot, Immunohistochemistry, Expressing

Journal: Antioxidants

Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

doi: 10.3390/antiox9080679

Figure Lengend Snippet: The summary diagram is demonstrated. Diabetes enhanced cardiovascular damage by suppression of antioxidant defense mechanisms including Nrf-2/HO-1, BAG3 and Bcl-2 and increased oxidative stress including vascular and cardiac reactive oxygen species (ROS). In response to myocardial IR injury, diabetes further decreased antioxidant defense mechanisms and enhanced oxidative stress/injury, leading to cardiovascular dysfunction and injury.

Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

Techniques: